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Mitochondrial fragmentation, elevated mitochondrial superoxide and respiratory supercomplexes disassembly is connected with the tamoxifen-resistant phenotype of breast cancer cells

https://www.sciencedirect.com/science/article/pii/S0891584919310044?via%3Dihub

Free Radical Biology and Medicine

Volume 143, 1 November 2019, Pages 510-521

Mitochondrial fragmentation, elevated mitochondrial superoxide and respiratory supercomplexes disassembly is connected with the tamoxifen-resistant phenotype of breast cancer cells

lVeronikaTomkováCristianSandoval-AcuñaNataliaTorrealbaJaroslavTruksa

https://doi.org/10.1016/j.freeradbiomed.2019.09.004Get rights and content

Highlights

Tamoxifen resistant cells exhibit altered mitochondrial structure and function.

Tamoxifen resistant cells show disassembly of respiratory supercomplexes.

Tamoxifen resistant cells reprogram their metabolism towards a glycolytic phenotype.

Tamoxifen resistant cells have fragmented mitochondria.

Tamoxifen resistant cells have increased mitochondrial ROS and antioxidant defense.

Abstract

Tamoxifen resistance remains a clinical obstacle in the treatment of hormone sensitive breast cancer. It has been reported that tamoxifen is able to target respiratory complex I within mitochondria. Therefore, we established two tamoxifen-resistant cell lines, MCF7 Tam5R and T47D Tam5R resistant to 5 μM tamoxifen and investigated whether tamoxifen-resistant cells exhibit mitochondrial changes which could help them survive the treatment. The function of mitochondria in this experimental model was evaluated in detail by studying i) the composition and activity of mitochondrial respiratory complexes; ii) respiration and glycolytic status; iii) mitochondrial distribution, dynamics and reactive oxygen species production. We show that Tam5R cells exhibit a significant decrease in mitochondrial respiration, low abundance of assembled mitochondrial respiratory supercomplexes, a more fragmented mitochondrial network connected with DRP1 Ser637 phosphorylation, higher glycolysis and sensitivity to 2-deoxyglucose. Tam5R cells also produce significantly higher levels of mitochondrial superoxide but at the same time increase their antioxidant defense (CAT, SOD2) through upregulation of SIRT3 and show phosphorylation of AMPK at Ser 485/491. Importantly, MCF7 ρ0 cells lacking functional mitochondria exhibit a markedly higher resistance to tamoxifen, supporting the role of mitochondria in tamoxifen resistance. We propose that reduced mitochondrial function and higher level of reactive oxygen species within mitochondria in concert with metabolic adaptations contribute to the phenotype of tamoxifen resistance.

Graphical abstract

© drbob 2018