367 (6478), 652-660

2020 Feb 7

An AMPK-caspase-6 Axis Controls Liver Damage in Nonalcoholic Steatohepatitis

Peng Zhao # 1Xiaoli Sun # 2Cynthia Chaggan 2Zhongji Liao 2Kai In Wong 2Feng He 3Seema Singh 4Rohit Loomba 4Michael Karin 3Joseph L Witztum 2Alan R Saltiel 1 3


PMID: 32029622

DOI: 10.1126/science.aay0542


Liver cell death has an essential role in nonalcoholic steatohepatitis (NASH). The activity of the energy sensor adenosine monophosphate (AMP)-activated protein kinase (AMPK) is repressed in NASH. Liver-specific AMPK knockout aggravated liver damage in mouse NASH models. AMPK phosphorylated proapoptotic caspase-6 protein to inhibit its activation, keeping hepatocyte apoptosis in check. Suppression of AMPK activity relieved this inhibition, rendering caspase-6 activated in human and mouse NASH. AMPK activation or caspase-6 inhibition, even after the onset of NASH, improved liver damage and fibrosis. Once phosphorylation was decreased, caspase-6 was activated by caspase-3 or -7. Active caspase-6 cleaved Bid to induce cytochrome c release, generating a feedforward loop that leads to hepatocyte death. Thus, the AMPK-caspase-6 axis regulates liver damage in NASH, implicating AMPK and caspase-6 as therapeutic targets.

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